The Interaction of Alphavirus E1 Protein with 1 Exogenous Domain Iii Defines Stages in Virus-membrane Fusion
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چکیده
1 EXOGENOUS DOMAIN III DEFINES STAGES IN VIRUS-MEMBRANE FUSION 2 3 4 5 Gleyder Roman-Sosa and Margaret Kielian* 6 Department of Cell Biology 7 Albert Einstein College of Medicine 8 Bronx, N.Y. 10461, USA 9 10 11 Present address: Department of Internal Medicine I, University Hospital of Ulm, Ulm D-89081, 12 Germany 13 14 Running Title: Stages in alphavirus membrane fusion 15 16 17 18 19 *Address correspondence to Margaret Kielian, Department of Cell Biology, Albert Einstein 20 College of Medicine, 1300 Morris Park Ave., Bronx, NY, 10461. Tel.: (718)430-3638. Fax: 21 (718) 430-8574. E-mail: [email protected]. 22 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Virol. doi:10.1128/JVI.05902-11 JVI Accepts, published online ahead of print on 21 September 2011
منابع مشابه
The interaction of alphavirus E1 protein with exogenous domain III defines stages in virus-membrane fusion.
Alphaviruses such as Semliki Forest virus (SFV) are enveloped viruses that infect cells through a low-pH-triggered membrane fusion reaction mediated by the transmembrane fusion protein E1. E1 drives fusion by insertion of its hydrophobic fusion loop into the cell membrane and refolding to a stable trimeric hairpin. In this postfusion conformation, the immunoglobulin-like domain III (DIII) and t...
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Alphaviruses are small enveloped RNA viruses that include important emerging human pathogens, such as chikungunya virus (CHIKV). These viruses infect cells via a low-pH-triggered membrane fusion reaction, making this step a potential target for antiviral therapies. The E1 fusion protein inserts into the target membrane, trimerizes, and refolds to a hairpin-like conformation in which the combina...
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Membrane fusion of the alphaviruses is mediated by the E1 protein, a class II virus membrane fusion protein. During fusion, E1 dissociates from its heterodimer interaction with the E2 protein and forms a target membrane-inserted E1 homotrimer. The structure of the homotrimer is that of a trimeric hairpin in which E1 domain III and the stem region fold back toward the target membrane-inserted fu...
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The E1 envelope protein of the alphavirus Semliki Forest virus (SFV) is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection. Like other class I and class II fusion proteins, during fusion E1 inserts into the target membrane and rearranges to form a trimeric hairpin structure. The postfusion structures of the alphavirus and flavivirus fusion proteins s...
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Alphaviruses and flaviviruses infect cells through low pH-dependent membrane fusion reactions mediated by their structurally similar viral fusion proteins. During fusion, these class II viral fusion proteins trimerize and refold to form hairpin-like structures, with the domain III and stem regions folded back toward the target membrane-inserted fusion peptides. We demonstrate that exogenous dom...
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تاریخ انتشار 2011